Systematic HPLC method development and robustness evaluation of 13 carbonyl DNPH derivatives


This video is about using DryLab software for method development, especially for the separation of 13 aldehyde and ketone derivatives. The standard mixture of 13 carbonyls is used in monitoring programs to determine aldehydes and ketones in air – indoor and outdoor. This is very important for human health because some aldehydes (for example formaldehyde) is a trigger for cancer and several autoimmune diseases. The determination of carbonyls in air samples will be performed usually with HPLC. The point is to become all 13 components (or maybe more, by the using of other standards) separated in one chromatogram. The separation of 13 carbonyls is a challange because of four unseparated peaks. To solve this problem we had to develop a new method. That’s why to optimize the separation conditions for all the 13 carbonyls, we used DryLab software. DryLab is computational prediction software. And this software is a very helpful tool for method development. By using this software in your HPLC daily work you can save your time, you can reduce the consumption of solvents, chemicals and energy. And actually you can perform so called “green HPLC”. The experimental part was performed on an AZURA Analytical system Consisting of AZURA pump P6.1L HPD, AZURA Detector DAD 6.1L, AZURA Autosampler AS 6.1L and Thermostat CT 2.1. As you can see in the chromatogram, the separation of all 13 carbonyls is reached.

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